Fig. 2. EYA protein expression during Drosophila oogenesis. Fluorescence confocal micrographs of wild-type egg chambers stained for GFP (ub-Cadherin-GFP) (green) and EYA (red) (A,B), or for EYA (green) and ß-galactosidase (red) (C-F). In C,E,F, expression of ß-galactosidase (red) from enhancer trap line A101 labels polar cells. In D, ß-galactosidase expression from enhancer trap line 93F is shown, which labels stalk cells. EYA protein is expressed in the germarium (A) and in all follicle cells except polar cells (A-C) and stalk cells (D) up to stage 8. (E) A stage 9 egg chamber in which EYA is excluded from the polar cells (arrows), but is expressed in migrating border cells. (F) EYA expression in a stage 10 egg chamber. Scale bars: in A, 10 µm for A; in B, 10 µm for B; in C, 10 µm for C-E; in F, 50 µm for F. Arrows in A,B indicate regions lacking EYA expression; arrows in C,E,F indicate polar cells.