Fig. 3. Loss of eya leads to ectopic polar cells in a cell autonomous fashion. Fluorescence confocal micrographs of epithelial follicle cells stained for GFP (green) (A-C) and for EYA (red in B), FAS3 (red in E) or ß-galactosidase from A101 (red in H,I). Co-localization appears yellow. GFP-positive cells are wild type, whereas the cells lacking GFP are eya mutant cells and are outlined in A-C. Scale bars: in A, 10 µm for A-C; in D, 10 µm for D-I.