Fig. 5. Interaction between CPC and R in yeast cells. (A) Schematic representation of the CPC and R proteins. Regions required for the interaction are indicated. (B) Interaction between CPC and R. (C) Interaction between intact CPC and truncated R. GBD-bound CPC and the series of GAD-bound R were used as bait and prey, respectively. (D) Interaction between truncated CPC and the N-terminal region of R. GBD-bound R of residues 1-371 and a series of GAD-bound CPC were used as bait and prey, respectively. The activity of ß-galactosidase was assayed using three to six independently transformed yeast lines.