Fig. 2. The Kinesin heavy chain is required for ooplasmic movement at stage 9. (A-D) Movement of GFP-Staufen particles in wild-type (A,B) and Khc mutant (C,D) egg chambers. Each image shows three consecutive time points of a time lapse movie that have been imported into the red, green and blue channels of a Photoshop file. In the nurse cell cytoplasm, the movement of GFP-Staufen particles in the Khc mutant is indistinguishable from that in wild type. In the ooplasm, however, most of the GFP-Staufen particles are static in the Khc mutants (white particles), and the ones that do move lie close to the ring canals. (E-J) Movement of particles that reflect 568 nm light in wild-type (E,F), Khc (G,H) and mago nashi (I,J) mutant egg chambers. In the Khc mutant, most of the particles in the oocyte are stationery (white particles), whereas all the particles in wild type and in mago nashi mutant oocytes move between time points. In staufen, barentsz and Tropomyosin II mutant oocytes, these particles also show the same movement as in wild type (I. M. P. and D. St J., unpublished). (K-P) Movement of particles that reflect 568 nm light in wild type (K-M) and Khc mutant (N-P) egg chambers. The oocytes were scanned either once (1; K,N) or in a continuous manner using the Kalman function of the confocal microscope for 15 (15; L, O) or 30 (30; M, P) scans, and the composite image is shown. Each scan lasts 7 seconds, so the images represent either 7 seconds (K,N), 105 seconds (L,O) or 210 seconds (M,P) of real time. The ooplasmic streaming observed in a stage 9 wild-type oocyte (K-M) is completely abolished in the Khc mutant oocyte (N-P). (Q,R) Quantitation of the movement of GFP-Staufen particles in wild-type and Khc mutant egg chambers. Movement of GFP-Staufen particles in wild-type (black boxes) and Khc mutant (grey boxes) egg chambers. The graphic shows the number of particles (x-axis) that move with a certain speed (y-axis) in both the nurse cells (Q) and the oocyte (R). In Khc mutant egg chambers, no movement of GFP-Staufen particles within the oocyte was observed, although the motion of these particles within the nurse cells was unaffected. This analysis does not consider the movements of the particles in the z-axis, but a more detailed analysis of the time lapse movies reveals that the particles move much less in this dimension.