Fig. 4. The Kinesin heavy chain and the Dynein heavy chain localise to the posterior pole of the oocyte. (A-C) Localisation of the KHC in wild-type (A), staufen (B) and Khc (C) mutant egg chambers. KHC localises to the posterior of the oocyte at the stage when oskar mRNA is localised to this pole (A). The posterior localisation of KHC does not depend on oskar mRNA, however, as KHC is also detected at the posterior in staufen mutant egg chambers (B). KHC staining is absent in the Khc null germline clones, demonstrating the specificity of the antibody (C). (D-F) Localisation of DHC to the posterior pole of wild-type (D), barentsz (E) and Khc (F) mutant oocytes. DHC localises to the posterior pole of the stage 9 oocyte (D). This localisation is completely abolished in the Khc mutant oocytes (F), but it is the same as in wild type in barentsz (E). DHC localisation is also the same as in wild type in staufen and mago nashi mutant egg chambers (I. M. P. and D. St J., unpublished). The mutant clones are marked by the absence of nuclear GFP.