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Fig. 5. Hematopoietic and endothelial cell analysis. Day 6 EB cells were subjected to three-color analyses for FLK1, CD4 and hematopoietic or endothelial cell markers. Cells gated on VE-cadherin+, CD31+, CD34+, Ter-119+ or CD45+ were analyzed for FLK1 and CD4 expression.