Fig. 6. Hematopoietic and endothelial cell lineage development. (A) Day 6 EB cells were sorted for FLK1⁺CD4^-, FLK1⁺CD4⁺, FLK1^-CD4⁺ and FLK1^-CD4^- and subjected to hematopoietic replating (5x10⁴ cells/ml). The hematopoietic colonies were counted after 5-7 days. Ery, erythroid; Mac, macrophage; Ery+Mac, bi-potential erythroid and macrophage colony. (B) FLK1⁺CD4^-, FLK1⁺CD4⁺, FLK1^-CD4⁺ and FLK1^-CD4^- cells sorted from day 6 EBs were cultured on type IV collagen coated plates for 4 days in the presence of VEGF (50 ng/ml). Four days later, the adherent cells were stained with PECAM1 (CD31) antibodies (right) or just treated with secondary antibody alone (left). FLK1^-CD4⁺ cells did not generate any adherent cells (not shown). (C) Day 8 EBs were sorted for FLK1^-CD4^-, FLK1⁺CD4^- and FLK1^-CD4⁺ cells and subjected to hematopoietic replating (5x10⁴ cells/ml). The colonies were counted 5-7 days later.