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Fig. 2. Confirmation of selected clones by RT-PCR. Individual clones were analyzed by RT-PCR in animal caps injected with 1 ng of Smad7 RNA and harvested at either stage 10.5 (gastrula; A) or stage 15 (neurula; B). Primers were designed to include either 5' or 3' UTR sequences. Ornithine decarboxylase (ODC) is used as a loading control and NCAM and NRP1 to control for neural induction by Smad7.