Fig. 2. hephaestus genetic mosaic wing phenotypes. (A) A dorsal anterior Df(3R)G45, f mitotic clone associated with ectopic dorsal anterior wing margin bristles. Df(3R)G45 mutant tissue is marked with forked (red arrowheads), and the adjacent twin is marked with bald (outlined in blue). Most of the ectopic margin is formed in heph⁺ tissue next to the clone. (B) A ventral anterior Df(3R)G45, M⁺ f mitotic clone associated with ectopic ventral anterior wing margin bristles (outlined in red). The growth disadvantage of Df(3R)G45 mutant tissue has been rescued with the Minute technique. The ectopic margin is induced next to the Df(3R)G45, M⁺ f mitotic clone when the clone boundary is close to the normal margin. (C) The margin inducing effect of heph clones is mainly non-autonomous. Df(3R)G45 mutant tissue has been genetically marked with yellow (arrowheads), which make up a minority of the ectopic bristles in this typical example. (D) heph mitotic clones induce ectopic wing margin only within a competent region close to the normal margin. Each red circle represents one of 129 patches of ectopic margin from 300 heph genetic mosaic wings. Only a fraction of the ectopic patches of bristles included Df(3R)G45, y marked ectopic bristles (12/58 patches from the anterior compartment where y can be reliably scored). (E,F,H) Examples of heph⁰³⁴²⁹ clones marked with pwn, outlined in red (dorsal) or blue (ventral). Loss of heph from dorsal or ventral clones is associated with ectopic wing margin (E,F) and/or wing margin nicks (F). Occasionally, heph clones that induce ectopic margin also induce small outgrowths and overgrowth of adjacent tissue.