Fig. 8. The intracellular localization of NICD is altered in hephaestus clones. A heph mosaic disc stained for NICD (A'-A''') or NECD (B'-B'''). Loss of heph is marked by loss of GFP from a P{Ubi-GFP} transgene (A,B). Each image is an XY projection representing one of three 10 µm apical (A',B') to basal (A''',B''') sections through each disc. The increased relative amount of NICD staining in heph⁰³⁴²⁹ clones appears to be inside of the mutant cells, not bound to the apical membrane. No corresponding increase is apparent using the NECD antibody. The position of the DV boundary is noted with an arrowhead, the position of heph clones are marked with arrows.