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Fig. 2. Expression analysis of Krm1 and Krm2 in the mouse. (A) Relative expression levels of mouse Krm1 and Krm2 in indicated tissues, as analysed by RT-PCR. Histone H4 was used as reference for sample normalisation. (B) Krm1 whole-mount in situ hybridisation of early (E8) and late (E8.5) headfold stage mouse embryos. (C) Whole-mount in situ hybridisation of E10.5 mouse embryos for Krm1 and Krm2 (top). Dissected anterior limb buds are shown in bottom panels, with arrows indicating staining in the apical ectodermal ridge (AER). br, branchial arches; f, forebrain; fl, forelimb; h, hindbrain; hl, hindlimb; m, midbrain; ms, mesanephros; np, nasal placode; ot, otic vesicle; ov, optic vesicle.