Fig. 2. Mobility shift assays for binding of VgRBP71 to VLE RNA. GST-VgRBP71 fusion protein (8 nM) and internally radiolabeled VLE RNA (1 nM) were incubated with 0, 1, 5, 10 or 25 nM unlabeled VLE RNA as a specific competitor (lanes 2-6, respectively), or 5, 25, 50 or 100 nM noncognate RNA (lanes 7-10, respectively). Lane 1 contains VLE RNA only.