Fig. 3. Temporal expression of VgRBP71. (A) Oocytes were separated into three groups according to the designated Dumont stages and total RNA isolated for northern blot analysis. Each lane contains 10 oocyte-equivalents of RNA. The positions of RNA size standards (nt) are indicated. (B) Staged oocytes or embryos were homogenized and two oocyte/embryo equivalents were run per lane in a western blot assay. The numbers below each lane refer to the developmental stage of the oocytes (Dumont, 1972) or embryos (Nieuwkoop and Faber, 1956). M indicates stage VI oocytes matured by treatment with progesterone.