Fig. 4. In vivo RNA binding assays. VgRBP71 was immunoprecipitated from whole cell extract. RNA in the precipitate was converted to cDNA by reverse transcription and amplified by PCR using gene-specific primers denoted by the bars above the relevant lanes of the agarose gels. For each mRNA tested, a standard was generated using total oocyte mRNA as the template for RT-PCR (PCR control). Control reactions included precipitation with protein A-Sepharose resin (—antibody) and protein A-Sepharose resin that had been adsorbed with pre-immune serum (Pre-immune serum).