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Fig. 6. Distribution of VgRBP71 during oogenesis. Staged oocytes were processed for immunocytochemical analysis using affinity purified antibody prepared against VgRBP71 and a secondary antibody conjugated with Alexa Fluor 568. Stage III through V oocytes are oriented with the vegetal pole at the lower left corner. The stage IV oocyte marked with an asterisk (IV*) was bisected along the animal-vegetal axis prior to immunochemical staining. The images marked VI(vg) and VI(an) are the vegetal and animal hemispheres, respectively, of a stage VI oocyte.