Fig. 2. Ebf1 inactivation induces a shift in thalamocortical and corticothalamic connections. Coronal hemisections of E16.5 brains where crystals of DiI (A-M) or DiI and DiA (N-P) were placed in several regions of the neocortex of controls (left) and Ebf1^-/- mutants (right). Schematic representations of a dorsal view of the brain (A,D,G,K,N) indicate the position of DiI and DiA crystals in the occipital (A,D), parietal (G), frontal (K), or parietal and occipital (N) neocortex. The insertion sites of DiI crystals are also visible in B and C (stars). The retroflexus tract, which can be used as a morphological landmark, is indicated by a white arrowhead (E,F,H,I,L,M,O,P). Open arrowheads indicate the medial boundary of the thalamic domain where cell bodies and axons are labeled in wild-type embryos (E,H,L,O). This wild-type boundary is indicated in Ebf1^-/- embryos (open arrowheads) and shows that position of the labeled thalamic domains are shifted medially (F,I,M,P). dTH, dorsal thalamus; Fr, frontal neocortex; IC, internal capsule; Ncx, neocortex; OB, olfactory bulb; Occ, occipital neocortex; Par, Parietal neocortex; Str, striatum.