Fig. 5. Ebf1 inactivation does not affect the molecular regionalization of the dorsal thalamus. Coronal hemisections of E16.5 heterozygous (left) and homozygous (right) Ebf1 mutant brains processed for in situ hybridization with the following probes: COUP-TFI (A,B), Cdh6 (C,D), Epha7 (E,F), Epha4 (G,H), Lhx9 (I,J), Gbx2 (K,L) and Sema6a (M,N). The expression domains of COUP-TF1, Lhx9 and Gbx2 cover large domains of the dorsal thalamus (A,I,K), Epha7 and Sema6a are excluded from dLGN (E,M), Cdh6 and Sema6a are low in medial nuclei (C,M), and Epha4 shows a lateromedial gradient within VB (G). Expression of these genes is not apparently perturbed by Ebf1 inactivation. Black arrowheads indicate the position of the retroflexus tract. A broken line indicates the pial surface of the thalamus in E,F,M,N. dLGN, dorsal lateral geniculate nucleus; dTH, dorsal thalamus; IC, internal capsule; Ncx, neocortex; VB, ventrobasal complex.