Fig. 2. Expression and activity of ephrin-A ligands in the E10 cerebellum. (A-F) RNA in situ hybridization (A,B,D,E) and affinity probe in situ (C,F) of whole mount cerebellum. Capital letters indicate domain names. (A-C) Dorsal is at the top. (D-F) Anterior is at the top. Expression of ephrin-A2 is strongest in domain C (lobules I-IXab), followed by domain A (lobules I-VIII) and E (lobules II-VII). ephrin-A5 is moderately expressed in domains A (lobules I-V), C (lobules I-V), D (lobules I-VIII) and F (lobules III-VIII). Total ligand activity detected with an EphA3-AP probe corresponds to the RNA expression patterns. (G) ephrin-A2 expression in a coronal section of the cerebellum (lobulus VII). Parasagittal pattern is seen in the Purkinje cell layer (pl). Asterisk, deep cerebellar nuclei. (H) A diagram of parasagittal domains in the cerebellum. Please note that expression patterns of ephrin-A2 and ephrin-A5 are shown only in domains A-C. Scale bars: 500 µm.