Fig. 3. Organotypic hindbrain culture. (A) The whole hindbrain containing the cerebellum and brainstem was isolated from E10 embryo. The cerebellum was cut along the dorsal midline (red line), and the both halves of the cerebellum were separated and turned over.

(B) Comparison of ephrin-A expression in domains A-C between the native cerebellum and explant. Dark purple: ephrin-A2(++), ephrin-A5(+) (rostral domain C). Light purple: ephrin-A2 (+), ephrin-A5(+) (rostral domain A). Light blue: ephrin-A2 (+ or ++), ephrin-A5(-) (middle parts of domains A and C).