Fig. 4. Anterograde tracing of IO axons. (A,D,G,J,M) DiI insertion sites in the IO
(red stars). Ventral views. IO areas are shown as in
Fig. 1P. (B,E,H,K,N) Projection
patterns of DiI-labeled axons (white arrowheads) in the contralateral side of
the cerebellum. (C,F,I,L,O) Domain patterns of ligand activity detected with
EphA3-AP. Black arrowheads indicate projection areas of DiI-labeled axons
shown by white arrowheads in B,E,H,K,N. (P) Scheme of cerebellar domains in
explant (see Fig. 3B). In each
panel, rostral is at the top. (A-C) Axons from the main part of Area L project
to the rostral parts of domain C. (D-F) The most caudal part of Area L maps to
the rostral domain A. (G-I) Area Ic maps to the rostral oval region of domain
B. (J-L) Area Ir maps to the middle region of domain C. (M-O) Area Ml maps to
the caudal domain B. Scale bar, 500 µm.