Fig. 1. Bioinformatics and categorization of S. mediterranea sequences.
(A) Organigram depicting the steps performed to produce a non-redundant
collection of S. mediterranea cDNA sequences (SmedDb). Bioinformatic
analyses compared the database against itself to identify and remove redundant
clones before sending sequences to the public databases. The GenBank protein
and nucleotide collections as well as the EST database (dbEST) were queried
using the BLAST algorithm. Sequences returning significant matches
(E
10-4) were subjected to annotation into functional categories
based on the identity/function of the GenBank match. (B) Distribution of
informative sequences by functional categories. For simplicity the cell
signaling category includes the cell/cell communication and internal signaling
categories. Metabolism is an amalgamation of the general metabolism,
mitochondria and protein metabolism categories. Visit
http://planaria.neuro.utah.edu
for a detailed categorization profile and access to sequences. (C)
Distribution by percentage of planarian genes displaying highest similarities
with members of either the vertebrates or invertebrates.