Fig. 2. Phenotypes of wing and leg bristles and of leg chemosensory neurons of wild type and Poxn null mutants. (A-D) Chemosensory wing and leg bristle phenotypes of wild type and Poxn null mutants. Bristles on the dorsal anterior wing margin (A,B) and the first male prothoracic tarsal segment (C,D) of wild type (A,C) and Poxn^M22-B5 mutants (B,D) are compared by bright field microscopy at a resolution of 20x and 25x magnification. (E-G) GFP expression in chemosensory neurons of wild-type and Poxn^M22-B5 male prothoracic legs. GFP expressing neurons are visualized in tarsal segments 4 and 5 of prothoracic legs of w; Poxn-Gal4-13-1-101 UAS-GFP/+ (E), w; Poxn^M22-B5; Poxn-Gal4-13-1-101 UAS-GFP/BasiK-109 (F) and w; Poxn^M22-B5; Poxn-Gal4-13-1-101 UAS-GFP (G) males by confocal fluorescence microscopy at a resolution of 40x magnification and with maximum projection of Z-stack. Note that tarsal segments 4 and 5 of Poxn^M22-B5 mutants endowed with (F) or without (G) the BasiK transgene are fused. Arrows indicate a cluster of four chemosensory neurons innervating a taste bristle (E) and a pair of bipolar neurons associated with a degenerated chemosensory organ (F,G), Dorsal side of legs is upwards (C,D) or towards the right (E-G).