Fig. 5. Sexually dimorphic pupal expression pattern of Poxn in the genital region and cuticular phenotype of Poxn^M22-B5 male genitalia. (A,B) GFP expression is analyzed in ventral views (anterior towards the left) of male (A) and female (B) w; Poxn-Gal4-14-1/TM6B pupae (48 hours APF) by fluorescence microscopy at a resolution of 10x magnification. (C) Details of GFP expression in the genital region of male pupae (72 hours APF), driven by three different Poxn-Gal4 constructs (Poxn-Gal4-581, Poxn-Gal4-13-1 and Poxn-Gal4-14-7; compare with Fig. 1B). (D) Frontal view (with dorsal side up) of cuticle preparations of male genitalia of wild type (w¹¹¹⁸; left) and Poxn mutant (right) under bright field microscopy at a resolution of 8x magnification. Arrows indicate anal plate (ap), clasper (cl), lateral plate (lp), penis (pe) and posterior lobe (pl). The four panels below compare details of the penis (left) and the posterior lobe, clasper and lateral plate (right) regions between wild type (wt) and Poxn mutants (Poxn) at a 2- to 2.5-fold greater magnification. Arrows indicate the wild-type penis (pe) and the penis apodeme (ad) in the Poxn mutant without penis. The four panels at the bottom illustrate, at the same magnification as the panels above, the rescue in Poxn mutants of the penis, but not posterior lobe and clasper, by two copies of EvK, which include the upstream genitalia enhancer absent from L1, and of the posterior lobe and clasper, but not penis, by two copies of L1, which include the intron genitalia enhancer not present in EvK. In the eight panels at the bottom, posterior is towards the right.