Fig. 1. Loss of hyd leads to ectopic differentiation and overgrowth in the eye disc. (A-C) Adult eyes; (A) wild type; (B) hyd^K3.5 clones have induced outgrowths of wild-type (white⁺) tissue; (C) hyd^K3.5 mutant clones generated in a Minute background. All surviving photoreceptors are white^- and therefore hyd mutant. (D-I) Third instar eye discs. Anterior is to the left and dorsal up in this and subsequent figures. (D-F) Anti-Elav staining in brown and X-gal staining, to show arm-lacZ expression, in blue. (D) Clones of wild-type tissue identified by lack of arm-lacZ expression. (E) Clones of hyd^K3.5 mutant cells identified by lack of arm-lacZ expression. Ectopic photoreceptors are visible in an anterior clone. (F) hyd^K3.5 clones generated in a Minute background. Remaining wild-type tissue is marked by arm-lacZ expression. (G,H) Anti-Ato staining in wild type (G) and in a disc containing an unmarked hyd^K3.5 clone (H), where a ring of ectopic Ato staining is visible anterior to the furrow. (I) An early third instar eye disc with hyd^K3.5 clones generated in a Minute background, stained with anti-Elav in red and anti-Ato in green. Ato is already restricted to single cells at the anterior of the disc.