Fig. 5. Ci, but not Smo, accumulates in the absence of hyd and hh. All panels show third instar eye discs with hyd^K3.5, hh^AC mutant clones (marked by lack of GFP expression in B,C,E,F). (A,C) Staining with an antibody to the C-terminal region of Ci. (D,F) Staining with anti-Smo. Arrows indicate examples of double mutant clones that do not accumulate Ci (C) and do not accumulate Smo (F).