Fig. 2. Neural crest gene expression. In situ hybridization was performed to confirm localization in premigratory and migratory neural crest. Owing to the caudal to rostral gradient in avian development, these stage 10 embryos are illustrative of multiple events in neural crest development, including induction (open neural plate), premigratory (dorsal neural tube) and migratory (cranial) neural crest. Gene expression in the embryos shown is representative of that observed in the pool of 4-to 16-somite embryos scored for each clone. White arrowheads indicate gene expression in neural folds, black arrowheads mark gene expression in migrating neural crest. (A-P) Dorsal view, anterior up. Labels A-P correspond to categories in Table 1. Expression of: (A) adenosylhomocysteinase 1 or 2; (B) palladin; (C) laminin 5; (D) hexokinase 1, isoform HKI; (E) KIF4a/chromokinesin; (F) Nopp140; (G) GCN20/ATP-binding cassette transporter F2; (H) tyrosine kinase receptor KLG; (I) MSE55; (J) dyskerin; (K) thimet oligopeptidase; (L) E12; (M) UV DNA damage binding protein 1; (N) cationic amino acid transporter 3; (O) D52-like 2; (P) human KIAA0147. (Q-X) Transverse sections, dorsal up. (Q) cathepsin D; (R) alanyl tRNA synthetase; (S) UV DNA damage binding protein; (T) CFR-associated protein; (U) paranemin; (V) laminin 5; (W) MSE55; (X) Nopp140.