Fig. 2. Expression of Disp overlaps with Hh expression in the mouse embryo. (A-H,J-L) Whole-mount in situ hybridization, using digoxigenin-labeled Shh and Disp riboprobes on wild-type mouse embryos at different stages of development from 7.75 to 10.5 dpc. (I,M-P) Section in situ hybridization using ³³P-UTP-labelled Disp and Ihh riboprobes on paraffin wax sections of wild-type mouse embryos from 9.5 to 16.5 dpc. (A,B) Lateral view of late streak, head process stage egg cylinder (7.75 dpc). Arrow in A indicates Shh expression in the node. (C,D) Ventral anterior view of head fold stage embryos just prior to somite formation (8 dpc). Arrowheads in D indicate Disp expression in cells immediately adjacent to the midline mesoderm and arrows indicate Disp expression at junctions between neural and surface ectoderm. (E,F) Stage showing 13-20 somites (9 dpc). Lateral view. (G,H) Stage showing 20-25 somites (9.5 dpc). Lateral view. (I) Cross-section of a wild-type 9.5 dpc mouse embryo at the forelimb level. Arrowhead indicates the notochord. (J) Dorsal view of H at the forelimb level. White arrows indicates Disp expression in the forelimb buds. (K,L) Stage showing 31-35 somite (10.5 dpc). Lateral view. (M) Cross-section of a wild-type 10.5 dpc mouse embryo at the forelimb level. (N) Cross-section of a wild-type 13.5 dpc mouse embryo through the thoracic cavity. (O,P) Longitudinal section through the metatarsal bones of the hindlimb of a wild-type 16.5 dpc mouse embryo. Phalanges (not shown) are to the right of the pictures. nt, neural tube; fp, floor plate; nc, notochord.