Fig. 6. Disp mutants exhibit multiple defects because of loss of Hh signaling. (A-D,Q-Z,AA-FF) Whole-mount in situ hybridization using digoxigenin-labeled riboprobes on wild-type (A,C,Q,S,U,W,Y,AA,CC,EE) and Disp^-/- (B,D,R,T,V,X,Z,BB,DD,FF) embryos at 9.5 dpc. All views are lateral except (Y,Z,AA,BB), which represent dorsal views at the forelimb level. (A,B) Brachury (T) expression; (C,D) Hnf3b expression; (Q,R) Pax1 expression; (S,T) Pax3 expression; (U,V) Myf5 expression; (W,X) myogenin expression; (Y,Z) Hand2 (dHand) expression; (AA,BB) Hoxd13 expression; (CC,DD) Fgf4 expression; (EE,FF) Fgf8 expression. Bracket in Y and arrow in Z indicate Hand2 expression in the limb, whereas the line next to the bracket indicates the extent of the limb bud viewed at this angle. Arrow in CC indicates Fgf4 expression in the posterior AER of the forelimb of a wild-type embryo. (E-P) Isotopic section in situ hybridization using ³³P-UTP-labeled riboprobes on paraffin sections of wild-type (E,G,I,K,M,O) and Disp^-/- (F,H,J,L,N,P) embryos at 9.5 dpc. (E,F,I) Cross-section at the hindbrain level; (G,H,J-P) cross-section at the forelimb level. (E,F) Hnf3b expression; (G,H) Pax3 expression; (I,J) Pax6 expression; (K,L) Dbx1 expression; (M,N) islet 1 expression; (O,P) Wnt3a expression. nt, neural tube; fp, floor plate; bp, branchial pouch.