Fig. 2. The Nek8 gene is mutated in jck mice. (A) Protein domain structure of the murine NEK8 protein (analysis carried out using the PFAM search server: http://pfam.wustl.edu/index.html). (B) Sequence changes at bp 1346 and 1348 in the jck mutant mouse result in a Gly-to-Val substitution at amino acid 448. (C) Conservation of the region containing the jck mutation across species. Mouse and zebrafish sequence determined as described in the text, human sequence is from multiple ESTs (Accession Numbers BF795289, BG755480 and AA076459), Sus scrofa sequence is from an EST (Accession Number BF189288), Tetraodon sequence is from a genomic survey sequence (Accession Number AL324795), Xenopus sequence is from an EST (Accession Number BE132147). (D) Northern analysis of Nek8 and Actb (ß actin) control. Br, brain; He, heart; Ki, kidney; Li, liver; Lu, lung; Mu, muscle; Sk, skin; Sl, small intestine; Sp, spleen; St, stomach; Te, testes; Th, thymus.