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Fig. 4. Histological analysis of mutant HFGa13-infected embryos. Hematoxylin and Eosin stained transverse (A-D; E7) and longitudinal (E-H; E6) sections of mutant HFGa13-infected (A,C,E,G) and control embryos (B,D,F,H). Ourentery is present in A. HFGa13-infected embryos show cloacal stenosis (CL*; C), atresia of the hindgut (HG*) anterior to the cloaca (CL; E), allantoic internalization (AL*; E) and defects in the cloaca-associated viscera, including more distal Müllerian duct (MN duct) atresia and cystic mesonephric (MN*) maldevelopment (G). Note the correct development of the more anterior gut structures. CE, ceca; GU, genitourinary; NC, notocord.