Fig. 5. HFGa13 affects expression of Fgf8, Hoxd13 and Bapx1. (A-E) Whole-mount in situ hybridization of control (left panel) and HFGa13-infected (right panel) embryos (arrows at malformed tail). (A) Fgf8 expression at E5, note absence of expression in the HFGa13 tail (red arrow) but normal expression in the hindlimb AER. (B) Bapx1 (expression) at E6. Bapx1 shows a diminished expression in the tail (red arrow) of the HFGa13-infected embryo but normal expression in the non-infected hindlimbs. (C) Wnt5a gene expression shows normal expression in the HFGa13-infected embryo at E7 (red arrow). (D) Hoxd13 expression at E6 shows strong downregulation in the tail (red arrow) of the HFGa13-infected embryo and normal expression in hindlimbs. (E) Shh expression at E4 is normal. Arrows indicate hindlimb and notochord.