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Fig. 4. Loss of Meis function disrupts hindbrain development. (A) {Delta}CPbx4 construct with amino acid positions indicated at the bottom. The red boxes indicate the PBC-A and PBC-B domains. The blue domain represents a biotin tag introduced at the C terminus. (B-D) {Delta}CPbx4 sequesters Meis3 in the cytoplasm. One- to two-cell stage embryos were injected with 300 pg of Myc{Delta}CPbx4 (B), MycMeis3 (C) or {Delta}CPbx4 +MycMeis3 (D), raised to 12 hpf and stained with anti-Myc antibody. (E-R) {Delta}CPbx4 affects gene expression in the hindbrain. One- to two-cell stage embryos were injected with 300 pg of {Delta}CPbx4 mRNA (F,H,J,L,N,P,R) or lacZ mRNA (E,G,I,K,M,O,Q), raised to 14 hpf (M,N) or 24 hpf (E-L,O-R) and analyzed by in situ hybridization for the genes indicated at the bottom of each panel. Black asterisks indicate the level of the otic vesicle on the right side of each embryo. Black asterisks on left side in Q, and R indicate rhombomere boundaries. Black triangle in R indicates region of strong pax6 expression. (S-W) {Delta}CPbx4 affects neuronal differentiation. One- to two-cell stage embryos were injected with 300 pg of {Delta}CPbx4 mRNA (S,U,V) or lacZ mRNA (T,W), raised to 48 hpf (S,T) or 28 hpf (U-W) and stained with anti-islet (S,T) or 3A10 (U-W) antibody. Black asterisks indicate the otic vesicle and rhombomeres are numbered on the left.