Fig. 3. Migratory pathways of neural crest cells within the sclerotome. (A) Neural crest cells from the wild-type embryo at E8.5 in vitro, immunostained with a neural crest cell-specific monoclonal antibody 4E9R (red) and anti-neuropilin 1 antibody (green). (B-E) Distribution of neural crest cells within the sclerotomes at the cervical level of E9.0 embryos. (B) A diagram indicating 9 regions of the sclerotome defined by different colors. (C-E) Histograms showing relative number (percentages) of neural crest cells contained within each region of the sclerotome shown in B, in the wild-type (C), neuropilin 1 homozygous mutant (D) and Sema3A homozygous mutant (E) embryos. d, dorsal; v, ventral; r, rostral; c, caudal; nt, neural tube; n, notochord; s, sclerotome. Scale bar: 50 µm in A.