Fig. 6. Effects of Sema3A on morphology of sympathetic ganglion explants and neurites. The sympathetic ganglion explants from the wild-type embryos at E12.5 were co-cultured with the control HEK293T cells (A-C) or the Sema3A-secreting HEK293T cells (D-F) (800 cells/cm² each). Phase-contrast micrographs of the explants at 12 hour (A,D), 24 hour (B,E) and 48 hour (C,F) in culture. Arrows indicate the HEK293T cells. Scale bar: 100 µm (A-F).