Fig. 1. Pdgfra+ and Sox10+ OLPs are generated from the Olig2+ pMN domain of ventral neuroepithelium in chicken. (A) E3 chicken spinal cords were double-labeled with anti-Nkx2.2 (brown) and Olig2 (blue). (B,C) E3-4 chicken spinal cords were double-stained with anti-Mnr2 (brown) and Olig2 (blue). Mnr+ motoneurons are produced from the Olig2+ domain dorsal to the Nkx2.2 domain. (D-G) Immediately adjacent chicken spinal cord sections from E7 (D), E8 (E,G) and E9 (F) were subjected to in situ hybridization with Pdgfra, Sox10 or Olig2 riboprobes. The ventral half from one side of the stained spinal cord was aligned with that from the same side of the adjacent cord. (H,I) Spinal cord from E7 chicken embryos were double-labeled with anti-Nkx2.2 (brown) and Sox10 (blue in H) or Pdgfra (blue in I). Sox10 and Pdgfra expression is located in the dorsal region of the Nkx2.2+ neuroepithelium (arrows).