Fig. 3. Double staining for Olig2 and Nkx2.2 in the hindbrain. Sections from E6 (A,B), E7 (C,D) and E8 (E,F) hindbrain tissues were subjected to anti-Nkx-2.2 immunostaining (in brown) followed by Olig2 in situ hybridization (in blue). Olig2 is only expressed in groups of migratory Nkx2.2+ OLP cells, but not in the ventricular cells. The representative double-positive cells are indicated by arrows, whereas the Nkx2.2+/Olig2- cells are represented by arrowheads.