Fig. 5. (A-E) Merging of the Olig2 domain and Nkx2.2 domain in mouse after the onset of emigration of Olig2+ OLPs. Sections from E12.5 (A,B), E13.5 (C,D) and E14.5 (E,F) were labeled by Nkx2.2 (left half in A,C,E), Olig2 (right half in A,C,E) or both (B,D,F). Nkx2.2 expression in the ventricular zone starts to expand dorsally at E13.5 and almost overlaps with the entire Olig2 domain at E14.5. (G-H) Co-expression of Olig2 and Nkx2.2 in OLPs in P1 mouse spinal cord. Spinal cord sections were double-stained with anti-Olig2 (in brown) and Nkx2.2 (in blue). Although double-labeled cells are present throughout the entire cord, pictures were taken only from the lateral (G) and ventral (H) regions. Olig2+ cells with strong Nkx2.2 staining are represented by arrows, while those with weak Nkx2.2 expression are represented by arrowheads.