Fig. 6. Co-expression of Olig2 and Nkx2.2 in dissociated spinal cord culture. The dorsal halves of E13.5 mouse spinal cords were isolated, dissociated and cultured on coverslips for 2 days (A-C) or 5 days (D-F). Cells were then simultaneously stained with anti-Nkx2.2 (green) and anti-Olig2 (red) by double immunofluorescence. The Olig2+ cells with strong Nkx2.2 expression are indicated by arrows, while those with weak Nkx2.2 expression are represented by arrowheads.