Fig. 5. Late cortical plate neurons in marginal zone of Dab1^p45/– hemizygotes. Dab1^p45/– and Dab1^p45/+ mice were crossed and pregnant females injected with BrdU on E12.5 (A,C) or E16.5 (B,D). (A,B) Dab1^p45/–, (C) Dab1^+/– and (D) Dab1^p45/+ littermates were sacrificed on P20 and BrdU-labeled nuclei detected by immunofluorescence. Note that, in both Dab1^p45/– and control, nuclei labeled at E12.5 are deep in layers IV-VI and nuclei labeled at E16.5 are shallow in layers II-III. Neurons labeled at E16.5 migrate into the marginal zone (MZ) in Dab1^p45/– but not in controls. Scale bar: 100 µm.