Fig. 6. Reelin response of cultured cerebral neurons. Neurons were cultured from Dab1p45/+ embryos, which express both p80 and p45, and treated with reelin-containing (Reln) or control (C) supernatant from 293T cells. Samples were immunoprecipitated with anti-Dab1(B3) and analyzed by SDS PAGE and western blotting with anti-Dab1(B3) (part A, lanes 6-10) or anti-phosphotyrosine (part A, lanes 1-5 and part B). (A) Incubation with serial fourfold dilutions of reelin-containing supernatant for 15 minutes, or (B) with undiluted reelin-containing supernatant for various times. Asterisks to the right of each panel mark nonspecific bands. Molecular mass standards were 95, 70, 54 and 47 kDa.