Fig. 6. PI3K/Dp110-dependent and -independent effects of Tkv^Q253D overexpression. (Left panels) Confocal images of Flip-out/Gal4 lateral clones of the indicated genotype visualized by GFP signal. (Right panel) FACS analysis of the same experiment. Nomenclature and color code as in Fig. 5. Clones co-expressing p60 and Tkv^QD contain as few cells as p60-expressing clones (arrows). The same phenotype was observed when cell death was blocked by co-expressing p35 (data not shown). These cells are slightly larger than p60 cells (size histogram) but show a wild-type cell cycle profile (DNA content histogram). Scale bar: 50 µm.