Fig. 7. Tkv^Q253D requires Vg to promote tissue growth but does not induce Vg expression. (A) Digital camera images of imaginal wing disc overexpressing GFP alone or with Tkv^QD in a vg mutant background. vg^83b27R heterozygous animals develop normally and wing discs reach normal size (upper left panel). Tkv^QD induces wing disc over-growth in a vg^83b27R heterozygous background, just as in a wild-type background (top right panel). The growth effect of Tkv^QD is blocked in a vg^83b27R homozygous animal (lower right panel); the size of the wing discs is similar to the size of vg^83b27R homozygous discs (lower left panel). (B) Digital camera images of Flip-out/Gal4 clones expressing GFP alone (lower left) or with Tkv^QD (lower right). Normal Vg expression pattern is shown in the upper left panel. Tkv^QD expression does not induce Vg protein upregulation, regardless of clone position (top right panel). Scale bars: 100 µm.