Fig. 6. Mutations in the RING-H2 finger motif of Deltex disrupted its homotypic interaction. UAS-Dx^full, -Dx^mRZF, -Dx^full+GST, and -Dx^mRZF+GST (see Fig. 2A,B) were expressed with pWA-GAL4 in Drosophila S2 cells. Deltex derivatives were detected by western blot with an anti-Deltex antibody. Lanes 1-7, the total cell extract that was added to the resin; lanes 8-14, eluants from the Glutathione-Sepharose 4B resin. Dx^full and Dx^mRZF are indicated by an arrowhead (90 kDa). Dx^full+GST and Dx^mRZF+GST are indicated by an arrow (120 kDa). Molecular weight markers are shown in kDa.