Fig. 2. Defects in bile duct differentiation in J1N2+/– mice. (A,B) Hematoxylin and Eosin-stained sections of livers at P8. Bile ducts (arrow,bd) are observed in the portal triads of the wild-type mouse (A), but not in the J1N2+/– mouse (B). (C,D) DBA-stained sections of livers at P6. DBA-positive cells form patent bile ducts (arrow) adjacent to the portal veins in wild-type mice (C). In J1N2+/– mice (D), DBA-positive cells (arrowheads) are present in small numbers adjacent to the portal veins, but these cells have not formed patent ducts. (E,F) WGA-stained sections of livers at P3. No differences in the numbers or distribution of WGA-positive hepatoblasts are observed. (G,H) Hematoxylin and eosin-stained sections of livers of 7-week old adult mice. Bile ducts (arrow) are observed in the portal triads of the wild-type mouse (G). In the J1N2+/– mouse (H), an abnormal proliferation of cells (outlined in yellow) is observed adjacent to the portal vein. bd, bile duct; ha, hepatic artery; pv, portal vein.