Fig. 7. rasp is required to produce active Hh protein. (A-F) A comparison of Hh target gene expression in wing discs that express different Hh transgenes and which are either heterozygous or homozygous for rasp^9B15. (A,B,E) A broken line marks the approximate position of the AP boundary. (A-C) en Gal4, UAS-GFP/UAS-Hh F ^HA; rasp^9B15/TM6 discs. (A) The domain of en Gal4 expression shown in green. The domain of anterior en expression can be detected (brackets). (B) Elevated Ptc protein levels can be detected in a narrow stripe anterior to the AP boundary (brackets). (C) Overlay of A and B. (D,E) en Gal4, UAS-GFP/UAS-Hh F ^HA; rasp^9B15/rasp^9B15 discs stained with anti-Ptc (red); en Gal4 expression shown in green. (D) High levels of anterior Ptc protein are not detected (compare C with D). Note that the anterior expression of en is also not detectable in these discs. (E) Higher magnification of disc shown in D. (F) UAS-Hh N/+; en Gal4, UAS-GFP/+_; rasp^9B15/ rasp^9B15 discs stained with anti-Ptc (red); en Gal4 expression shown in green. High levels of anterior Ptc protein are not detected.