Fig. 6. Reduced Notch signaling results in the decrease of Slug expression. Electroporation of GFP (A,B), GFP+Delta1^stu (dominant-negative Delta1, C,D), GFP+chick Numb (E,F) was performed on stage 5 embryo, and the embryos were cultured for 9 hours until stage 9. Transfected areas are visualized by the fluorescence of GFP overlaid on bright field image of electroporated live embryos (A,C,E). Slug expression in the head fold (B,D,F) is shown at higher magnification, corresponding to the boxed areas of A,C,E, respectively. Reduction of Notch signaling results in the decrease of the Slug expression in the head fold (arrowheads in D,F).