Fig. 9. Exogenous Bmp4 rescues the reduction of Slug expression by Delta1^stu, but not the reduction by CNIC^C89. Electroporation of GFP (A,B), GFP+Noggin (C,D), GFP+Bmp4 (E,F), GFP+Bmp4 +CNIC^C89 (G,H), GFP+Bmp4+Delta1^stu (I,J), was performed on stage 5 embryo, and the embryos were cultured for 9 hours until stage 9. Transfected areas are visualized by the fluorescence of GFP overlaid on bright field image of electroporated live embryos (A,C,E,G,I). Misexpression of Noggin reduces Slug expression (D, see also the section in the inset, arrowhead), while overexpression of Bmp4 results in the expansion of Slug domain (F, see also the section in the inset, arrowheads). The loss of Slug expression by CNIC^C89 cannot be rescued by the co-expression of Bmp4 (H, see also the section in the inset, arrowhead). The decrease of Slug expression by Delta1^stu (see Fig. 6D) is rescued by the co-expression of Bmp4 (J, see also the section in the inset).