Fig. 3. Phenotype of 18.5 dpc fetuses inheriting the (ChßGI)₂. (A) RT-PCR SNuPE assays. +/–(P) and +/+ lanes; top row is presumptive inactive allele. –(M)/+ lanes (in boxes) (these fetuses were obtained from the reciprocal mating, hence bottom row is presumptive inactive allele). Parental origin of alleles is on the right. Value above each band is the amount of RNA contributed by the presumptive inactive allele as a percent of the total. (B) (a) +/+ fetus; (b) +/–(P) fetus. (C) Northern blots. Values under bands are the mean relative amounts of RNA standardized according to Gapd mRNA. All four Igf2 transcripts were reduced to the same extent in +/–(P) mice (only the largest transcript is shown). Number of embryos used: liver, one in all lanes; kidney, two in +/+ and –(M)/+ lanes, and four in +/–(P) lanes. (D) Northern blots, 11.5 dpc midgestation embryos. One embryo was used for each lane. Other details as in C.