Fig. 6. Different intracellular localisations of TBP and TLF. (A) Confocal images of sectioned seminiferous tubules stained with antibodies against TLF (upper panels) or TBP (lower panels) as indicated. A single representative section is shown. RS, haploid round spermatids; ES, elongating spermatids; C, the chromocenter. The lower panel shows perinuclear TBP detected in late stage elongated spermatids. (B) Confocal sections of pachytene spermatocytes (PS) stained with TLF or TBP as indicated. In all panels the middle and right hand images show the corresponding Hoechst stained DNA and merged images respectively. 400x magnification.