Fig. 5. Aos is repressed in the intervein tissue by the activity of Cic. (A) Wild-type and (B) cic²/bwk¹¹ third larval instar wing imaginal discs carrying the aos-lacZ reporter stained with anti ß-Gal. The aos reporter gene is expressed throughout the wing pouch in the mutant. (C,D) Wild-type and cic²/bwk¹¹ wings (24-30 hours APF) carrying the aos-lacZ reporter stained as in A,B. Homogeneous levels of the aos reporter are found throughout the wing blade in the mutant. (E,F) Third larval instar imaginal wing disc expressing the aos-lacZ reporter and containing cic² M⁺ mutant clones marked as cells lacking GFP (green in F). Staining with anti-ß-gal (red in F) shows that aos-lacZ is expressed autonomously in all the cic mutant cells.